Changes in the specificity of antibodies by site-specific mutagenesis followed by random mutagenesis
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چکیده
منابع مشابه
Changes in the specificity of antibodies by site-specific mutagenesis followed by random mutagenesis.
The specificity for 11-deoxycortisol (11-DOC) of a monoclonal antibody (mAb), designated SCET, was changed to specificity for cortisol (CS) by site-specific mutagenesis followed by random mutagenesis. The Fab form of SCET was expressed on the surface of a phage. During the first step, mutations were introduced at 14 amino acid positions in three complementarity-determining regions (CDRs) of the...
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Overproduction of Clavulanic Acid by UV Mutagenesis of Streptomyces clavuligerus
Clavulanic acid is produced industrially by fermentation of Streptomyces clavuligerus and researches have increased its production by strain improvement, recombinant DNA technology, and media composition and growth condition optimization. The main objective of this study was to increase the level of clavulanic acid production from Streptomyces clavuligerus (DSM 738), using UV irradiation. After...
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ژورنال
عنوان ژورنال: Protein Engineering, Design and Selection
سال: 1999
ISSN: 1741-0134,1741-0126
DOI: 10.1093/protein/12.5.407